沃兴德 卢德赵 唐利华; 李毅; 施孟如; 沃立科; 杨贞;

目的:应用凝胶内差异显示电泳技术和质谱技术研究温补肾阳药对肾上腺切除的肾阳虚大鼠肝线粒体蛋白质组的影响。方法:通过切除两侧肾上腺造成肾阳虚模型大鼠和温补肾阳药治疗大鼠肝线粒体蛋白质经荧光染料Cy3、Cy5标记,与等量Cy2标记的内标混合后在同一胶中进行电泳分离,经不同光激发后扫描得到不同样品的蛋白质组图谱。利用DeCyder软件进行差异蛋白质组分析,筛选出18个差异蛋白质进行质谱鉴定,其中15个蛋白质得到鉴定。结果:经温补肾阳药治疗后,肾阳虚动物氨甲酰磷酸合成酶、过氧化氢酶、二氢硫辛酸脱氢酶、热休克蛋白60、亚硫酸氧化酶、ATP合成酶、醛脱氢酶、丙酮酸脱氢酶、3-巯基丙酮酸转硫酶、腺苷酸激酶同工酶5、谷胱甘肽过氧化物酶表达均增高。结论:温补肾阳药可能通过加快糖、脂类、蛋白质和核酸分解代谢,加快ATP合成以及清除自由基和过氧化物,维持线粒体膜稳定性,从而起到治疗肾阳虚的作用。
    Objective:Two-dimensional difference gel electrophoresis and mass spectrum was used to study the effects of Warm and Tonify Kidney-Yang Herbs on the Liver Mitochondria Proteome of the adrenalectomized Kidney-Yang deficiency Rats.Methods:Every liver mitochondria protein sample from the kidney-yang deficiency rats and the kidney-yang deficiency rats treated with warm and tonify kidney-yang herbs was labeled with Cy3 or Cy5 randomly,Each Cy3-labeled sample and Cy5-labeled sample were mixed on the same 2-D gel ...